Improving cattle genetics with in vitro embryo production technology

Livestock scientists from ILRI and the Clinical Studies Department of the University of Nairobi (UON) recently succeeded in breeding Kenya’s first test-tube calf using a technique called in vitro embryo production (IVEP). IVEP makes it possible to rapidly multiply and breed genetically superior cattle within a short generation interval.
Why is this important?
For several reasons. First, livestock is the fastest growing sub-sector in the world, as increasing trends of 114% in demand for meat and 133% for milk attest. To improve on food security, it is essential to double livestock production in the developing world by 2020. IVEP is clearly one of the most efficient ways to accomplish this.

Second, let’s consider the problem of environmental impact. Doubling livestock production through traditional breeding techniques increases pressure on natural resources—water, land and biodiversity. So the need for enhanced efficiency without degrading natural resources is urgent. Again, IVEP, which requires only laboratory equipment in the production process, comes to the rescue.

Third, there is the biodiversity issue. Matching genotypes to environment is crucial. Scientists need to take several factors into consideration—among them adaptation, tolerance for disease, tolerance for new environments and alignment to market development. Although plenty of genetic diversity exists, thus far we’ve done little with it. Once more, IVEP could be the answer.

Fourth, IVEP has significant commercial potential because farmers can rent their best cows as donors and their lower-quality cows as surrogates.

Most importantly, we need to look closely at the constraints faced by small-scale livestock keepers.

  • Cattle genotypes and production environments, as often as not, do not match. Result:  low productivity.
  • Heifer replacement programs take a long time and are rarely done properly. Result: supply is low, prices are high.
  • Sex ratios are often disadvantageous. Result: too many males and high production costs.
  • The commercial relevance of many indigenous breeds is not optimised. Result: farmers incur unsupportable losses.
  • Programs for breed conservation and preservation are often improper. Result: some breeds are threatened by extinction and no gene pool for replacement exists.

IVEP does not—and should not—completely replace traditional reproductive technologies such as conventional embryo transfer (ET) and artificial insemination. Each of these techniques has its place, and each of them utilizes tissues, embryos and semen for improvement and reconstruction of cattle breeds. The difference is that while the traditional ET techniques involve more animals and are wholly done in the field, IVEP is undertaken in the lab and involves fewer surrogate animals in the field. IVEP eliminates the tedious steps of synchronizing donor cows.

Specifically, IVEP technology as a breeding tool has the distinct advantage of maximizing utilization of appropriate dam and sire genotypes by:

  • increasing efficiency of multiplication in breeding;
  • permitting  determination of sex of the offspring; and
  • permitting pre-testing of actual fertility status of the bull.

Thus, while natural mating or artificial insemination are necessarily slow and inefficient, producing only 10-15 offspring per life span of a cow …

…IVEP can produce up to 300 offspring per life span.

The SIFET Project: a successful IVEP program
The Sexed semen in-vitro fertilization and embryo transfer (SIFET) project was designed to exploit and promote the potential of applying IVEP reproductive technique to:

  • develop, multiply and disseminate female crossbreeds that appropriately match with production environment;
  • provide a system to preserve top bovine genotypes in cases of accidental culling in a recycle-like scheme (slaughterhouse collection); and
  • identify, multiply and conserve selected superior desirable breed traits.

The project involved collecting ovaries from slaughter houses or picking ovum from live cows. When the genetic material is brought to the lab, oocytes with high developmental competence are selected and morphological evaluation done. Once the ideal oocytes are identified, they are matured in vitro for 22-24 hours. The subsequent in vitro fertilization process is conducted for a period of 18-22 hours with a high sperm concentration. The fertilization itself requires removal of seminal plasma and extenders, separation of motile sperms from dead ones and induction of sperm capacitation. Once the embryos are formed, they are cultured in the lab for 7 days and then transferred to surrogates.

A conception rate of about 40% has been achieved, with calves born without abnormalities.

Conclusions

  1. IVEP technology is feasible in Kenya.
  2. Commercialization of the process should be facilitated as soon as supportive policies and proper legal/regulatory frameworks are in place

Challenges
Poor field heat detections leading to poor uterine synchrony and lower conceptions are concerns, as is the high genotype variability characteristic of animals brought to slaughterhouses.

Way forward and prospects
Looking ahead, the collaborating scientists anticipate bringing ovum pick-up (OPU) and cryopreservation into the picture as well as capacity building.

Clearly, such programs can help match breeds to appropriate production systems to ensure sound breeding programs. Where and when necessary, new breeds can be introduced within a relatively short period of time. Above all, embryos are far easier to transport across continents than live animals.

Through IVEP technology and well-planned crossbreeding programs such as SIFET that integrate the use of indigenous cows as donors and surrogates while using semen from appropriate (more productive and reasonably adapted) dairy breeds such as Jerseys, F1 heifers suited to the smallholder farmers’ conditions can be produced.

Niche markets for the technology and its F1 products should be further explored and exploited, notably with regard to the potential of forestalling the threat to key wildlife species.

Acknowledgements
Funding for the project was made available by Heifer Project International. UON provided the technical team and recipient animals. Administration and laboratory facilities were provided by ILRI. The cooperation of the abattoirs (the source of ovaries) and the animal owners are gratefully acknowledged. The capacity building program through a joint CNPq grant for the Embrapa-UON-ILRI partnership, as well as support from Dr Luiz Carmago and Dr Joao Viana of Embrapa, are highly appreciated.

The collaborating scientists are Mwai Okeyo, Henry Mutembei and Bridgit Syombua from ILRI; and  Erastus Mutiga, Victor Tsuma and Henry Mutembei from Clinical Studies, UON.

For more information, contact Dr Okeyo Mwai, Animal Geneticist/Breeder, Biotechnology Theme, ILRI, at o.mwai@cgiar.org.

The promise of science for development

Doherty Lecture by Dame Bridget Ogilvie urges Africa not to follow Britain’s example but rather to help its science and scientists to flourish.

The brain drain can be stopped and redressed in Britain as well as Africa. But two things must happen: one, governments must do more to create an environment where science can flourish; two, scientists and technicians must be nurtured, developed and rewarded for their talents and contributions.

This was the key message of Dame Bridget Ogilvie who delivered the Peter Doherty Distinguished Lecture on 24 November 2004, at the Nairobi headquarters of the International Livestock Research Institute (ILRI).

Doherty Lecture Announcement Poster

Dame Bridget emphasized the importance of people development and career development in research institutes. She placed great importance on training and developing the scientists and graduates who do the work. The success of any research institute is dependent on the expertise of staff.

‘My first and my most important message to anyone interested in accelerating scientific innovation is the need to ensure that the appropriately trained, talented and supported workforce is available as nothing can happen without it.  My experience suggests that this is neither easy nor cheap to achieve, but it is essential.’

She recalled her work at the Wellcome Trust, which she directed from 1991 to 1998, and how difficult it was to entice veterinary graduates into research. Salary differentials of academic and practicing scientists created further challenges, so polices were introduced to increase salaries of the academics to stem any brain-drain. The UK has already witnessed this, and she urged Africans to learn from these mistakes and take proactive stops to nurture their intellectual capital.

In closing, Dame Bridget said: ‘The extraordinary scientific revolution…..will continue to bring great benefits to the public.  However, this will occur where governments not only provide the funds that are necessary but also exhibit leadership by providing the regulatory, social, fiscal and working conditions that make a nation an attractive place in which scientists and the innovative industries that depend on them can function well.’

ILRI’s Distinguished Lecture series is named after Australian Peter Doherty, 1996 Nobel Laureate in Medicine or Physiology, who from 1986 to 1992 chaired the program committee of the board of trustees of ILRI’s predecessor, the International Laboratory for Research on Animal Diseases (ILRAD).
Dame Ogilvie’s lecture is now available as a book, The Promise of Science for Development.

The electronic version is available in PDF format.


Printed copies are available from ILRI’s Addis and Nairobi InfoCentres: Requests can be made to InfoCentre Team

Innovation Africa Symposium – November 20-23, Kampala, Uganda

Call for papers from research and development actors.
 
This November, in Kampala, internationally recognized experts on innovation systems will share their latest thinking with agricultural researchers and development partners. The Innovation Africa Symposium is now calling for papers and exhibit contributions. The closing date for receipt of abstracts for the Innovation Africa Symposium has been extended from 15 August 2006 to 1 September 2006.

Contributions are invited in two forms:

  1. Symposium papers that draw on diverse fields and disciplines of the social, agricultural and natural resource sciences, and present good practice in studying and enhancing the process of innovation for effective agricultural research, development and education.
  2. Marketplace exhibits that can be in the form of posters, videos, slides, photographs, websites, maps, group interactions (e.g. participatory theatre) and other lively ways of showing how work on innovation systems is being conducted in Africa and elsewhere. The marketplace will feature the creativity and experience of farmers, farmer organisations and other local entrepreneurs and institutions.

Abstracts for papers and proposals for marketplace exhibits should be submitted by 1 September 2006 to the Innovation Africa Symposium Secretariat (innovationafrica@cgiar.org) with copies to p.sanginga@cgiar.org and ann.waters-bayer@etcnl.nl.

More details about guidelines for abstracts, deadlines for papers, symposium costs and registration can be found in the Symposium Brochure and the Call for Contributions on the Symposium website: www.innovationafrica.net and are also available below.

Symposium Brochure (PDF file; 340 KB)
Call for Contributions (PDF file; 62 KB)

This symposium is organized by five organizations: the International Centre for Tropical Agriculture (CIAT, Colombia), the International Food Policy Research Institute (IFPRI, the division of International Service for National Agricultural Research, Ethiopia), the International Livestock Research Institute (ILRI, Africa), the International Institute for Rural Reconstruction (IIRR, Kenya), and the NGO PROLINNOVA (PROmoting Local INNOVAtion, Netherlands).

The collective power of smallholder farmers

Kenya's new Dairy Development Policy aims to bring Kenya's estimated 39,000 informal milk traders into the formal sector.

BBC World Service began to broadcast a Kenya dairy story on 6 April 2006, the same day Kenya’s Minister for Livestock and Fisheries Development, Joseph Munyao, presented a new Dairy Development Policy in Nairobi – the final step before the new Policy and accompanying Dairy Development Bill are presented to Kenya’s Parliament.

Kenya’s new Dairy Policy now broadly reflects the approach the International Livestock Research Institute (ILRI) and its partners have advocated over the past several years – the need to engage with and develop the country’s hugely important informal milk market, which provides a livelihood to an estimated 1.8 million smallholder households.

The new Dairy Policy now clearly acknowledges the role of the informal market actors in the development of the sector. The policy states that it will ‘facilitate the transformation of the informal milk trade towards formalisation’. Specific measures mentioned include development of low-cost and appropriate technologies for small investors, training programmes on safe milk handling, efforts to improve the standards of milk processing in the informal sector, provision of incentives for improved milk handling, and establishment of a supportive milk dealer certification system. The Dairy Policy also announces that the Kenya Dairy Board functions will be streamlined and steps will be taken so that the Board regulates itself and is managed by its stakeholders.

The new Dairy Policy is a huge step forward in a struggle that has been ongoing since 1998, when Kenya’s big milk buyer (Kenya Co-operative Creameries) went into liquidation. Various attempts were subsequently made to oust smallholders from the market, including media campaigns advising that unpasteurized (‘raw’) milk was unsafe and should not be consumed. The BBC Kenya Dairy Story, broadcast on its World Service, tells how the smallholders, supported by ILRI and partners, fought back, and how they are now being brought into the formal milk market. The Kenya Dairy Story was broadcast from 6-13 April 2006.

Small is Beautiful – The Kenya Dairy Story
Kenyans love their milk. Most of the 4 billion litres consumed there each year is produced by smallholders with a couple of cows, and sold house-to-house by thousands of street hawkers and doorstep milkmen. But this whole milk business was under threat. In the third edition of the One Planet series (on BBC World Service) which is sharing small business success, Susie Emmett discovers how the farmers and traders fought back to keep the milk flowing.


Listen to a recording of the BBC World Service broadcast produced by WRENmedia.

Note: The latest numbers

Some of the numbers quoted in this BBC World Service broadcast ‘Small is beautiful’ have been obtained from much earlier estimates. These figures, however, grossly understate the true size and extent of Kenya’s milk sector. SDP has provided recalculated figures, which more accurately reflect the picture in Kenya today.

1. Smallholder dairy farms recalculates to be 1.8 million (up from 800,000)

The estimated 800,000 smallholder farms has been widely cited for many years, during which time Kenya’s population has grown significantly. SDP recalculates the number of smallholders to be 1.8 million.

2. Milk hawkers recalculated to be 39,650 (up from 30,000)

SDP recalculates the number of small milk vendors in Kenya to be 39,650.

3. Number of dairy cattle recalculated to be 6.7 million (up from 3 million)
There are concerns about the reliability of the official cattle figures for Kenya; no livestock census has been conducted for decades and the methods used to estimate cattle numbers are imprecise. A conservative estimate of the size of the national dairy herd using detailed SDP survey data suggests that there are about 6.7 million dairy cattle (2.7 million high grade and 4 million crosses) owned by 1.8 million rural smallholder farms mainly in the Kenyan Highlands. This projected cattle population is more than twice the officially reported figure of 3 million for the national herds.

4. Total milk produced recalculated to be 4 billion litres per annum (up from 3 billion)
Based on SDP’s recalculated cattle projections above, SDP recalculates total milk production in the rural highlands to be an estimated 4 billion litres per annum.

5. Annual consumption of milk recalculated to be 145 litres per person (up from 100 litres)
SDP recalculates annual milk consumption by Kenyans to be 145 litres per person, making Kenyans amongst the highest milk consumers in the developing world. The rural areas have an estimated population of about 14.5 million people. Assuming that the estimated 9.6 million people living in the urban areas mainly depend on milk from the high potential areas, and that 13 percent of production goes to calf feed or spoilage loss, milk availability from the highlands was estimated to be about 145 litres per person per year. Previously, milk consumption in Central and Rift Valley provinces, which are important milk production areas, has been estimated to be between 144 and 152 litres per person per year.

Source: SDP Policy Brief No.10.

Highlights from the Kenya Dairy Story broadcast by BBC World Service:
In Kenya, a knock on the door means the milkman is here – today as everyday – delivering fresh raw milk to one of his many grateful customers. “I like this milk because this one comes daily, and it is fresh and good, that’s why I like it” says one of his customers.


This milk vendor serves approximately 60 customers, and sells approximately 100 litres of raw milk, each and every day. In Kenya, more than 30,000 milk hawkers [recalculated in August 2006 to be 39,650] are out daily on their bicycles or pushing carts to deliver nutritious milk from 800,000 small dairy farms [recalculated in August 2006 to have risen to 1.8 million].

Despite all its success, this whole wonderful milk business was under threat. The government here, as in so many countries, decided that small-scale farmers and traders couldn’t supply milk as safely as large farms and dairies. So, how come the hawkers are still in business, as are the smallholder dairy farmers whose milk they sell?

Kenya has three million improved dairy cattle [recalculated in August 2006 to have risen to 6.7 million] – that’s the largest dairy herd in Africa – and most of them are on small farms. Almost half the three billion litres of milk they produce are sold direct from small farms to households nearby. So the question is, can smallholder farmers produce clean, safe milk? Research by ILRI and others says yes – and changed lives because of it.

But what about that other all-important business criteria: efficiency; are the smallholder dairy farmers here in Kenya efficient? Steve Staal, agricultural economist at ILRI, says research shows smallholder dairy farmers are actually very competitive. ‘Indeed, small-scale mixed crop-and-livestock production is likely to be a more sustainable way to intensify agricultural production in environmental terms than large-scale industrial livestock production’, says Staal.

The biggest threat to smallholders came a few years ago when the one big buyer in Kenya – The Kenya Cooperative Creameries – went bust. Government policies did not recognize the small-scale operators and thus they were deemed to be operating illegally. Amos Omore is part of the team at ILRI trying to boost dairy incomes for the poor. He remembers how big dairy business was not going to let Kenya’s 800,000 [1.8 million] new milk entrepreneurs get in their way. It was a clash between big and small.

Kenya’s official ban on milk hawking was based on the milk not being safe. The nation’s newspapers carried many such stories. In the face of this scare-mongering, researchers got researching. David Mwangi at the Kenya Agricultural Research Institute (KARI), says that the public health risks being talked about were minimal – ‘almost not there’.On average, Kenyans drink 100 litres of milk a year, [recalculated in August 2006 to be 144–152 litres of milk a year] making them among the highest milk consumers in the developing world. But they don’t drink milk as it comes. ILRI’s Omore says the research showed that most consumers boil fresh milk before drinking it, which makes it as safe as pasteurized milk.

Gathering this evidence was a huge step. Using that evidence to change policy and mindsets was another.


KARI’s Mwangi says, ‘We worked with advocacy groups and hosted a high-profile meeting. And we had facts to table there.’ This research helped lead to the Kenya Dairy Board approving training for smallholder milk producers and traders. That’s important: most members of smallholder dairy cooperatives depend on their milk money to educate their children. Furthermore, easing restrictions against small traders helps poor customers because of the price of processed milk is beyond the means of most poor people here.’

John Kutwa, a former ILRI technician in the dairy team, says that ‘small’ characterizes most of Kenya’s milk business. ‘It’s small farmers selling to small traders and processors who deliver to small (poor) consumers.’


ILRI’s Amos Omore has been battling on behalf of smallholder milk producers and sellers since their troubles began. And he’s battling still. ‘Right now the policy environment has shifted towards small-scale traders’ he says. But the change is not yet complete. Small businesses are important but often overlooked.

‘Changing policies takes time’, he says, ‘and so does changing attitudes. In this country in the 1960s and 70s, it was always assumed that development projects should be “mega” to achieve some quantum leap in development. But if you look at development holistically – in terms of employment, in terms of nutrition, in terms of cash flow – these are the stepping stones that allow people to move from one living standard to the next. Small is beautiful!  Small should not be sacrificed at the altar of large-scale businesses that often fail.’

Fodder innovations for smallholders in India

Improved fodder varieties and technologies offer better quality feed all year through for highly valued livestock in Hyderabad.

The online magazine New Agriculturist published the following article in its March 2006 issue;
http://www.new-agri.co.uk/06-2/focuson/focuson5.html.

Further information on this topic can be found on ILRI's website and its 2004 annual report;
https://www.ilri.org/home.asp?CCID=61&SID=1.

smallholders in IndiaHyderabad is one of India's fastest growing cities. The local markets in the central square sprawl onto the road, sellingcredit:Stevie Mann/ILRI everything from black pearls and embroidered rugs to plastic key rings. Fresh fruit stalls steadied on bicycles cluster by the side of the main road as motorbikes and rickshaws weave their paths through the chaos. Like the markets, the Indian economy is thriving and in the farming state of Andhra Pradesh, where Hyderabad is the capital, livestock produce is at the heart of development. Throughout India, livestock are highly valued for their agricultural products and buffalo, cattle, goats and pigs are the most important source of livelihood for poorer people in the state. Livestock supply daily food and milk, as well as draft power and manure, and the dairy industry provides valued employment for the poor, especially women. But many farmers cannot produce quality fodder – or enough of it – which prevents them from taking advantage of increased market opportunities and demand.


Rapid population growth, particularly in urban areas, has increased demand for produce such as milk and meat. However, the population expansion also means that there is little land available to support fodder production, in addition to the area needed for food crops. Family plots are divided and reduced over generations, making many plots too small to sustain livestock. And while public land is often used as a grazing area for livestock among marginal communities, the areas are shrinking. Consequently, over 40 per cent of fodder resources in India come from crop residues and are of poor quality.

Food and fodder

To help the poor in Andhra Pradesh benefit from India's livestock revolution, the International Livestock Research Institute (ILRI) is co-ordinating a project under the Systemwide Livestock Programme (SLP) enabling smallholders to build on their assets by exploiting the growing market for livestock products. The aim of this work has been to improve fodder varieties and technologies in order to provide livestock with more and better quality feed throughout the year. Under the project, over 500 farmers from 47 villages have tested seed delivery systems and evaluated fodder and feed technologies. This process has included the farmers evaluating their own 'food-feed' crops (those that provide both grain for human consumption and fodder for livestock) and management systems, testing varieties provided by the research team, and evaluating researcher-managed demonstration trials. During the trials, farmers consistently found improved varieties to be superior to local cultivars.


smallholders in IndiaThe project has also supported seed supply for forage crops, since these are scarcely available from the commercial seed companies operating in Andhra Pradesh. Young people and women's self-help groups from several villages have been trained in seed multiplication and distribution, and village seed banks have been given support in sourcing germplasm from the public sector. In 2005 over 350 farmers attended field days and seed multiplication plots to learn about forage seed production.

Including fodder in food crop development

Credit:Stevie Mann/ILRIA third focus has been in raising awareness about fodder quality in India's crop improvement programmes. Nutritional studies have shown a wide variability in digestibility in stover from different sorghum varieties. But is has also been shown that high yield in food (grain or legume pods), can be compatible with high quality and quantity in crop residue. As a result, indicators of stover quality have now been incorporated into the sorghum and millet breeding programmes of the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), and India's National Research Centre for Sorghum has included stover quality in its release criteria for the last two years.


There is more work to be done, with the researchers continuing to find new ways of working with partners to increase the uptake of the technologies. A particular challenge will be to involve more women and minority groups in testing and evaluating new seed varieties. Partnerships with the private sector are also being explored, to investigate employment opportunities and further broaden seed choice and variety. Private sector dairy companies are being encouraged to promote fodder seeds in locations not served by dairy co-operatives. Looking more widely, the research team are hopeful that lessons from this project can be applied internationally.

Electronic version of important poverty mapping book for Uganda available here

An electronic version of an important book, Where are the Poor? Mapping Patterns of Well-Being in Uganda, is now available.

Uganda has some of the poorest people in the world. For the first time, the question Where are the poor in Uganda? can be answered, as a result of sophisticated poverty maps developed by the Uganda Bureau of Statistics and the International Livestock Research Institute (ILRI). These maps provide facts and figures on poverty and inequality by region, district and county, highlighting where the poorest are located and estimating the numbers of poor and levels of poverty. These maps are important because they can be used to ensure that resources are targeted at those most in need.

If you are interested in viewing the entire electronic version of the book, click open:

If you are interested in viewing this book by chapter, go to:

To view the maps from the Atlas of Estimated Measures of Poverty Below the Regional Level: 1992 Poverty Maps, go to:

To view the maps from the Atlas of Estimated Measures of Poverty Below the Regional Level: 1999 Poverty Maps and the Change in Poverty from 1992 to 1999, go to: